K. J. Somaiya

In the current study precise and accurate reverse phase liquid chromatographic method has been developed and validated for simultaneous estimation of simvastatin and metformin as the reversed phase HPLC method is most appropriate for the assessment of both the ingredient simultaneously. The method was developed using Jasco Auto sampler HPLC of 50mM Sodium Dihydrogen Phosphate Monohydrate at pH 3.0: Acetonitrile (30:70 v/v). Analysis was performed using UV-Visible detector and the eluents were monitored at 235 nm. The retention time of simvastatin and metformin was observed at 7.650min and 2.858min respectively. The method was validated with respect to linearity, robustness, precision and accuracy. The projected method was successfully applied for the simultaneous quantitative determination.