This paper gives brief idea about wireless based operation and control of electron gun for medical LINAC. A medical LINAC that use to destroy tumors using high-energy beams. The electron gun is a source of electron beams. Todays leading technologies, such as ZigBee, Bluetooth, GPRS/GSM can help to make wireless operation and control which gives reliability and security as well reduce the wiring and cost. The aim of this project is to make operation and control of electron gun through a long distance i. e. without any physical connection. To achieve this aim, Zig-Bee wireless technology is used. For this, on one side, zigbee interfaced microcontroller hardware is required and on another side zigbee interfaced personal computer is required. Personal computer consists of graphical user interface program by using this user can send the commands to the microcontroller and microcontroller is also programmed so accordingly it indicates the status of microcontroller and communicate with user.